In this work we provide examples of how transcription assays can be used to determine the mechanism of regulation by transcription elongation/termination factor NusA, and how the …

In this article, we present an overview of transcription, including requirements of in vitro transcription reactions and a comparison of conventional vs. large scale RNA synthesis.

Sep 20, 2016 · In vitro transcription assays have been developed and widely used for many years to study the molecular mechanisms involved in transcription. This process requires multi …

Learn the principles of in vitro transcription, how it is performed and applications for this common RNA synthesis technique in drug discovery.

The Assay Genie Transcription Factor Activity Assay (ELISA) Kit contains components necessary for detection of active transcription factors in eukaryotic nuclear or cell lysates. This particular …

We will describe the basic parameters for the use of in vitro transcription systems for the analysis of transcription termination and its control, and will also discuss variations on the assay system …

Herein, we describe a new, non-radioactive, robust and reproducible eukaryotic in vitro transcription assay that has been established in our laboratory. Upon transcription, the newly …

A nuclear run-on assay measures the amount of Pol II that is either actively transcribing or is “transcriptionally engaged,” tightly associated with DNA in preparation for transcription.

Transcription factors interact at promoters to modulate the transcription of genes. This chapter describes three in vitro methods that can be used to monitor their activity: transcript assays, …

Apr 4, 2016 · Transcription factors (TFs) play central roles in the regulation of gene expression through binding to specific DNA sequences, and may influence multiple transcription …