Reversed-phase HPLC came into routine laboratory use in the late 1970s and combines high resolving power and recovery with relative ease and speed of operation. Most small peptides and proteins ...

Figure 1: Schematic representation of the continuous FFE apparatus coupled off-line to RP-HPLC. Hydroxypropyl ... rack systems (Greiner) Reversed-phase column (Brownlee RP-300 cartridge; 100 ...

The latest edition of "Column Watch" features our annual review of new liquid chromatography columns and accessories, ...

Reversed-phase HPLC or Ultra-high Performance Liquid Chromatography (UHPLC) is a commonly used separation mode. It provides dynamic retention of compounds possessing hydrophobic and organic ...

In this article we will discuss the HPLC analysis of ONs using the Thermo ... The Thermo Scientific™ DNAPac™ RP column is a reversed-phase column designed for the high resolution separation ...

Normally reverse phase HPLC is the method of choice in these cases as denaturing conditions are required. Reverse Phase Agilent ZORBAX Rapid Resolution High Definition (RRHD) 300SB-C18 columns (1. ...

Normal phase and reversed phase chromatography can often be performed on the same HPLC system however some system modifications may be required to convert systems in the standard configuration to the ...

Assessing the effects of the structure of the analyte and in particular: how molecular weight and polarity of analytes are related to the most suitable type of HPLC and why reversed phase mode is ...

24, 2015 Accepted: Dec. 2, 2015 Prior to this report, there were just two ways to increase the amount of sample that could be purified in a single run by preparative reversed-phase high-performance ...

Below, the differences between HPLC and IC are outlined. In HPLC, separation is based on hydrophobic interactions (reversed phase HPLC) or hydrophilic interactions (normal-phase HPLC) of the ...