The amount of input target protein sample to use depends on the detection limit of the assay, but 10% of the amount added to each binding reaction (3 μl) is usually sufficient. The unbound target ...
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0.1 M KCl, 0.5 mM phenylmethylsulfonyl fluoride (PMSF), 1 mM DTT. Add PMSF and DTT just before use. The amounts of recombinant protein and the DNA template must be titrated. MgCl 2 is typically ...